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. 2012 Aug 7;7(8):e42288. doi: 10.1371/journal.pone.0042288

Table 3. Primers for limited mutagenesis of the m01s loop DE.

Position Change to Codon* Comment Diversity
Y hydrophobic amino acid KHT Unexpected “D” and “S” will be introduced 6
N neutral hydrophilic amino acid WMC An unexpected “Y” will be introduced 4
S neutral hydrophilic amino acid WMC An unexpected “Y” will be introduced 4
T neutral hydrophilic amino acid WMC An unexpected “Y” will be introduced 4
Y hydrophobic amino acid KHT Unexpected “D” and “S” will be introduced 6
*

K  =  T + G, H  =  A + T + C, W  =  A + T, M  =  A + C.