Figure 1. Expression and purification of unlabeled RGD-hirudin and labeled RGD-hirudin were analyzed by 15% SDS-PAGE.

Lane M, molecular mass standards (116 to 14.4 kDa ). a. Loading was 10 µL of culture supernatant of unlabeled RGD-hirudin for 0 to 20 h after methanol induction. b. Loading was 10 µL of culture supernatant of labeled RGD-hirudin for 0 to 20 h after methanol induction. c. Loading was 10 µL of the purified unlabeled RGD-hirudin (Lane 1) and labeled RGD-hirudin (Lane 2).