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. 2012 Aug 7;7(8):e42207. doi: 10.1371/journal.pone.0042207

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© 2012 Huang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a. HPLC analysis of purified ¹⁵N, ¹³C-labeled RGD-hirudin showed the purity was up to 98%. b. Mass spectrometry of the purified proteins for unlabeled RGD-hirudin showed its molecular weight was 7021 Da c. The molecular weight of ¹⁵N, ¹³C-labeled RGD-hirudin was 7387 Da The total labeling ratio is more than 98%.