Table 3. 15N, 13C and 1H Resonance Assignments for r-RGD-Hirudin at pH 7.4 and 25°C.
| Residue | Chemical shift (ppm) | |||||
| 15N | C | Cβ | C’ | NH | Hα | |
| V1 | 60.0 | 33.1 | 176.3 | 3.53 | ||
| V2 | 125.8 | 61.6 | 32.6 | 175.3 | 8.32 | 4.03 |
| Y3 | 125.9 | 57.1 | 40.0 | 175.2 | 8.33 | 5.00 |
| T4 | 111.9 | 59.4 | 71.9 | 176,2 | 9.05 | 4.67 |
| D5 | 121.1 | 55.8 | 40.3 | 176.9 | 8.54 | 4.86 |
| C6 | 121.1 | 55.3 | 39.9 | 175.0 | 8.95 | 4.63 |
| T7 | 113.8 | 61.4 | 70.5 | 174.4 | 9.50 | 4.40 |
| E8 | 120.3 | 54.6 | 33.2 | 174.9 | 7.39 | 4.57 |
| S9 | 119.7 | 59.6 | 63.3 | 175.9 | 9.01 | 4.86 |
| G10 | 112.8 | 44.2 | 173.3 | 9.72 | 4.59, 3.34 | |
| Q11 | 120.6 | 54.8 | 32.7 | 175.6 | 7.42 | 5.30 |
| N12 | 118.7 | 51.9 | 39.9 | 174.5 | 8.21 | 4.54 |
| L13 | 113.6 | 55.3 | 37.8 | 175.2 | 9.23 | 3.25 |
| C14 | 109.2 | 53.1 | 43.6 | 172.0 | 7.30 | 4.98 |
| L15 | 119.8 | 54.9 | 39.2 | 174.0 | 9.08 | 4.26 |
| C16 | 126.1 | 56.6 | 40.6 | 172.3 | 7.46 | 4.75 |
| E17 | 120.7 | 54.8 | 27.8 | 175.8 | 8.67 | 4.46 |
| G18 | 116.5 | 46.3 | 174.5 | 8.48 | 3.95, 3.61 | |
| S19 | 120.1 | 57.6 | 62.9 | 173.4 | 8.70 | 4.39 |
| N20 | 119.2 | 52.7 | 39.2 | 176.4 | 7.97 | 4.81 |
| V21 | 128.7 | 63.3 | 31.7 | 174.4 | 9.07 | 3.40 |
| C22 | 129.3 | 53.0 | 37.3 | 174.6 | 8.47 | 5.12 |
| G23 | 116.7 | 44.0 | 172.2 | 9.16 | 4.03, 3.98 | |
| Q24 | 117.8 | 58.0 | 28.3 | 177.1 | 8.50 | 4.03 |
| G25 | 114.2 | 44.3 | 172.7 | 9.12 | 4.24, 3.66 | |
| N26 | 120.3 | 51.8 | 44.0 | 172.3 | 8.07 | 5.51 |
| K27 | 112.2 | 54.6 | 35.5 | 173.1 | 9.32 | 4.45 |
| C28 | 120.8 | 53.9 | 42.5 | 174.1 | 9.11 | 5.38 |
| I29 | 131.8 | 59.2 | 36.7 | 176.6 | 9.86 | 4.28 |
| L30 | 130.0 | 54.9 | 41.0 | 178.3 | 8.36 | 4.13 |
| G31 | 109.8 | 44.4 | 173.1 | 9.01 | 3.82 3.42, | |
| R32 | 121.5 | 54.9 | 31.4 | 177.1 | 8.11 | 4.51 |
| G33 | 113.0 | 46.5 | 174.9 | 8.87 | 4.69 3.81 | |
| D34 | 125.3 | 54.3 | 40.6 | 176.4 | 8.69 | 4.62 |
| S35 | 116.2 | 58.1 | 64.6 | 173.4 | 7.98 | 4.52 |
| K36 | 120.7 | 55.0 | 33.6 | 176.9 | 8.36 | 4.42 |
| N37 | 120.8 | 52.7 | 38.8 | 175.1 | 8.70 | 4.76 |
| Q38 | 118.0 | 54.6 | 33.5 | 173.1 | 8.45 | 4.73 |
| C39 | 127.9 | 54.8 | 38.8 | 174.6 | 10.3 | 5.42 |
| V40 | 124.4 | 59.2 | 34.7 | 174.8 | 9.27 | 4.88 |
| T41 | 116.1 | 63.7 | 69.7 | 174.7 | 8.28 | 4.46 |
| G42 | 114.2 | 44.1 | 171.3 | 8.27 | 4.12, 3.61 | |
| E43 | 119.6 | 56.8 | 29.2 | 176.4 | 8.09 | 4.18 |
| G44 | 113.8 | 44.3 | 172.7 | 7.85 | 4.58, 3.55 | |
| T45 | 115.8 | 57.8 | 70.6 | 180.8 | 8.19 | 4.85 |
| P46 | 62.4 | 31.9 | 175.1 | 4.62 | ||
| K47 | 125.4 | 55.0 | 32.9 | 174.9 | 8.42 | 4.38 |
| P48 | 63.7 | 31.9 | 177.0 | 4.46 | ||
| Q49 | 121.9 | 55.5 | 29.5 | 175.4 | 8.63 | 4.32 |
| S50 | 121.7 | 8.29 | 4.37 | |||
| H51 | 112.9 | 8.17 | 4.72 | |||
| N52 | 122.8 | 53.4 | 38.7 | 175.0 | 8.38 | 4.64 |
| Q53 | 121.3 | 56.6 | 28.9 | 176.4 | 8.54 | 4.76 |
| G54 | 109.9 | 44.4 | 173.8 | 8.44 | 3.90, 3.84 | |
| D55 | 120.4 | 54.5 | 40.9 | 175.7 | 8.17 | 4.12 |
| F56 | 119.4 | 57.3 | 39.6 | 175.1 | 8.04 | 4.61 |
| E57 | 124.5 | 53.7 | 29.9 | 173.4 | 8.18 | 4.41 |
| P58 | 62.4 | 31.7 | 176.6 | 4.38 | ||
| I59 | 123.3 | 58.6 | 38.4 | 175.0 | 8.25 | 4.41 |
| P60 | 63.4 | 31.9 | 177.0 | 4.38 | ||
| E61 | 121.2 | 56.4 | 30.0 | 176.3 | 8.58 | 4.09 |
| D62 | 120.8 | 54.3 | 40.9 | 175.7 | 8.29 | 4.53 |
| A63 | 123.9 | 52.3 | 19.0 | 177.2 | 8.05 | 4.24 |
| Y64 | 119.6 | 57.7 | 38.7 | 175.4 | 8.13 | 4. 57 |
| D65 | 122.7 | 54.0 | 41.2 | 174.9 | 8.25 | 4.61 |
| E66 | 126.0 | 58.0 | 31.1 | 176.6 | 7.83 | 3.96 |