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. 2012 Aug 7;10(8):e1001367. doi: 10.1371/journal.pbio.1001367

Figure 3. PER and TIM in head extracts of flies expressing CUL-3K717R or Cul-3 RNAi.

Figure 3

Flies were entrained for 3 d in LD, then transferred to DD. Gray and black bars indicate subjective day and subjective night, respectively. (A) TIM (top, Tris-acetate gel) and PER (bottom, Tris-glycine gel) Western blot analysis of head extracts from flies collected the first and second days of DD. Phosphorylated (P-) and hypo-phosphorylated forms of PER and TIM are indicated. (B) TIM (left) and PER (right) Western blots (both Tris-glycine gels) of head extracts from flies collected the second day of DD. Two (Cul-3RNAi) or three (Cul-3K717R) independent Western blots were done for each condition with very similar results.