Figure 6. PER and TIM proteins in flies Cul-3^K717R and slmb^m flies.

(A) Anti-TIM (top) and anti-PER (bottom) Western blots of head extracts. Flies were entrained 3 d in LD and transferred to DD for collection during the first and second day of DD. Gray and black bars indicate subjective day and subjective night, respectively. (B) Anti-PER Western blots of head extracts. Flies were entrained 3 d in LD and collected the fourth day. White and black bars indicate day and night, respectively. (C, D) Anti-PER (C) and anti-TIM (D) Western blots of head extracts tim⁰ and per⁰ backgrounds, respectively. Flies were entrained for 3 d in LD and transferred to DD for collection at CT27. Vertical dashes indicate that the two surrounding slots were not contiguous on the gel. Two to three independent Western blots were done for each condition with very similar results.