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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: FEBS Lett. 2012 May 11;586(17):2575–2579. doi: 10.1016/j.febslet.2012.05.002

Figure 2. Constitutive rate of tyrosine dephosphorylation in cells is high.

Figure 2

Human 293T cells were treated with 50 micromolar pervanadate for the indicated times to inhibit cellular tyrosine phosphatases. Cell lysates were then immunoblotted with anti-phosphotyrosine antibody (pTyr). Levels of tyrosine phosphorylation rise rapidly in pervanadate-treated cells. Lysates were also immunoblotted with anti-actin as a loading control (bottom). Positions of molecular weight markers are indicated on left (in kDa).