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. Author manuscript; available in PMC: 2013 Aug 14.
Published in final edited form as: FEBS Lett. 2012 May 11;586(17):2575–2579. doi: 10.1016/j.febslet.2012.05.002

Figure 4. Surface rebinding affects apparent off-rates.

Figure 4

Binding is illustrated for an SH2 domain to phosphorylated sites (red circles) on an RTK on the membrane. For simple chemical kinetics, the amount of binding of the SH2 domain is determined by the on-rate and off-rate for the bimolecular interaction (left). If density of binding sites and on-rate are high, however, the domain may “hop” from site to site on the membrane (dotted arrows) before finally escaping. The dwell-time on the membrane is much longer than predicted by the simple off-rate.