Table 1.
Target Gene | Primer Sequence |
---|---|
Angiogenin | Forward 5′- CAG CAT GTG GAC CCT CAG GT -3′ Reverse 5′- TGA AGC GTT TGC ACT GGA CA -3′ |
Annexin-1 | Forward 5′-CGG ATG TTG CTG CCT TGC AC -3′ Reverse 5′-CCT TGA TCT GCT GGC GCT GA -3′ |
bFGF | Forward 5′-TGT GTG CCA ACC GGT ACC TT-3′ Reverse 5′-TTC CAG TCG TTC AAA GAA GAA ACA -3′ |
CD31 | Forward 5′-CAG TGT CCC CAG AAG CAA AAT CA -3′ Reverse 5′- AGA GAG CAA TGA TCA CTC CGA TG -3′ |
CD44 exon6 common |
Reverse 5′ GAT CCA TGA GTC ACA GTG CG -3′ |
CD44 exon5 | Forward 5′- GCC TAC TGG AGA TCA GGA TG -3′ |
CD44v10 | Forward 5′- CTA AGA GCG GCG CTA AAG AT -3′ |
CD44v9 | Forward 5′- CAC AGA GTC ATT CTC AGA AC -3′ |
CD44v8 | Forward 5′- ATA CAG ACT CCA GTC ATA GT -3′ |
CD44v7 | Forward 5′- CTT CGG CCC ACA ACA ACC AT -3′ |
CD44v6 | Forward 5′- CTC CTA ATA GTA CAG CAG AA -3′ |
CD44v5 | Forward 5′- ATA GAC AGA ATC AGC ACC AG -3′ |
CD44v4 | Forward 5′- TTG CAA GTA CTC CAC GGG TT -3′ |
CD44v3 | Forward 5′- GTA CGG AGT CAA ATA CCA AC -3′ |
CD44v2 | Forward 5′- TGA TGA CCA CCC CTG AAA CA -3′ |
CD44v1 | Forward 5′- TTG CCT CAA CTG TGC ACT CA -3′ |
Endoglin | Forward 5′-CTC CAG CTG CGG TGG TGT GT -3′ Reverse 5′- TGA GTA GCA CGG GGC TGC AT-3′ |
GAPDH | Forward 5'-CCT GCA CCA CCA ACT GCT TA-3' Reverse 5'-CAT GAG TCC TTC CAC GAT ACC A-3' |
Heparanase | Forward 5'- GGG GCC GGA TGG ATT ACT TT-3' Reverse 5' -CCA TGA AAA ACC CGT CTC CA-3' |
HGF | Forward 5′-GGG ACG GTA TCC ATC ACT AAG A -3′ Reverse 5′-CTT TAC CGC GAT AGC TCG AA-3′ |
MMP2 | Forward 5′-GCA TCG CTC AGA TCC GTG GT-3′ Reverse 5′-GAA TGT GGC CAC CAG CAA GG-3′ |
MMP8 | Forward 5′-GCT TAC AGG GAA CCC AGC ACC T-3′ Reverse 5′-GGG CCC AGT AGG TTG GAT GG-3′ |
MMP9 | Forward 5′-TAA AGG CCG CTC GGA TGG TT-3′ Reverse 5′-CCA ACT ACG GTC GCG TCC AC -3′ |
NRPI | Forward 5′- TGT CGC TAT GAC CGG CTG GAT -3′ Reverse 5′- TCC GGC CAG TTT TCT GC -3′ |
TIE-2 | Forward 5′-ACT TGG AGC CGC GGA CTG AC -3 Reverse 5′- AGG CCC ATG CCC TTC TC -3′ |
TWEAK | Forward 5′-CCG AGC TAT TGC AGC CCA TT-3′ Reverse 5′-GCC ACT CAC TGT CCC ATC CA-3′ |
VE-Cadherin | Forward 5′- AGC CCA GGC GGG TGT CAG -3′ Reverse 5′- CCG CCT CCG CAG GAT GAT-3′ |
VEGF-A | Forward 5′- GCC AGC ACA TAG AGA GAA TGA GC-3′ Reverse 5′- CAA GGC TCA CAG TGA TTT TCT GG-3′ |