Figure 1. MUC1-C suppresses AR expression.
A. Lysates from LNCaP and DU145 cells were immunoblotted with the indicated antibodies. B and C. LNCaP (B) and LAPC4 (C) cells were stably transfected to express GFP or MUC1-C. Lysates were immunoblotted with the indicated antibodies. D and E. Total RNA from the indicated LNCaP (D) and LAPC4 (E) cells was analyzed by RT-PCR for AR and, as a control, GAPDH mRNA levels.