Skip to main content
. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Prostate. 2012 Apr 2;72(15):1659–1668. doi: 10.1002/pros.22519

Figure 4. MUC1-C associates with AR on the PSA promoter.

Figure 4

A. Schematic representation of the PSA gene promoter with positioning of the androgen response element (ARE). B and C. Soluble chromatin from LNCaP/GFP and LNCaP/MUC1-C cells was precipitated with anti-AR or anti-MUC1-C and then analyzed for PSA promoter sequences. The final DNA samples were amplified by PCR (B) and qPCR (C) with pairs of primers for the AR binding region (−300 to −1) or a control region (CR; −4893 to −4675). The results (mean±SD of three determinations) are expressed as the relative fold enrichment compared to that obtained with the IgG control (C). D and E. Soluble chromatin from LNCaP/GFP and LNCaP/MUC1-C cells was precipitated with anti-AR. The anti-AR precipitates were released, reimmunoprecipitated with anti-MUC1-C, and then analyzed for PSA promoter sequences. The final DNA samples were amplified by PCR (D) and qPCR (E). The results (mean±SD of three determinations) are expressed as the relative fold enrichment compared to that obtained with the IgG control (right).