Fig. 3.

Thioridazine promotes pro-apoptotic signaling through inhibition of p-Akt activation. a Effect of thioridazine and other PI3K inhibitors on PI3K activity was assayed by immunoblotting for total PI3K and phosphorylated PI3K. The reaction products were visualized using autoradiography and liquid scintillation counting. b HeLa and HEC-1-A cells were treated with each chemical drugs and analyzed for the indicated proteins by immunoblotting for total Akt/phosphorylated Akt (p-Ser 473 and p-Thr 308), GSK-3β/phosphorylated-GSK-3β (p-Ser 9) and 4E-BP1/phosphorylated-4E-BP1. Results shown are representative of three independent experiments