Figure 1.

Concentration-dependent inhibition of ASIC3 channels by synthetic APETx2 toxin. (A) CHO cells expressing rat ASIC3 channels were incubated 30 s with or without APETx2 in the pH 7.4 bath solution then exposed for 5 s to a pH 6.0 solution with or without 500 nM APETx2. (B) Concentration dependence of ASIC3 current inhibition by APETx2. The inhibition was calculated for each toxin concentration from the measured ASIC3 peak current induced by pH 6, normalized to the peak current in the absence of toxin. Stimulations were performed every 35 s for 5 s, to allow recovery of channels. The dashed line represents a Hill fit to the experimental data points (n > 3).