Figure 4.

The rate of H₂S consumption in liver tissue (A) and H₂³⁵S consumption in brain tissue (B) incubated with vehicle (0.1% EtOH) alone or with 3 µM stigmatellin. Stigmatellin reduced H₂S consumption by the liver but did not alter H₂³⁵S consumption by brain tissue. Brain tissue was further analysed for the conversion of H₂³⁵S to [³⁵S]-sulphate (C) and [³⁵S]-thiosulphate (D). Stigmatellin had no significant effect on the conversion of H₂³⁵S to [³⁵S]-sulphate or [³⁵S]-thiosulphate. Data are the mean ± SEM values for three (liver) or five (brain) independent experiments run in duplicate. *P < 0.05 compared with vehicle-treated control; paired t-test.