Figure 5.

The proportion of H₂³⁵S delivered to brain tissue with vehicle (0.1% EtOH) or 3 µM stigmatellin that was recovered as either [³⁵S]-sulphate, [³⁵S]-thiosulphate or H₂³⁵S, all three of these molecules, unidentified ³⁵S recovered from the filter (>30 kDa) or HPLC column or a combination of the three identified molecules, and the ³⁵S that was not identified. Nearly all of the supplied H₂³⁵S was recovered as H₂³⁵S when added to syringes containing the incubation solution without tissue (solution alone). Brain tissue reduced the proportion of H₂³⁵S recovered as H₂³⁵S and increased the proportions of H₂³⁵S recovered as [³⁵S]-sulphate, [³⁵S]-thiosulphate, and unidentified [³⁵S] (*P < 0.05 compared with solution alone, Kruskal–Wallis test followed by Dunn's test). Stigmatellin (3 µM) had no effect on any of these measurements. Data are the mean ± SEM proportions for 4–10 observations.