Table 2.

Operational model of allosterism parameters for the functional interaction of Org 274179-0 with TSH and M22 at the TSH receptor

Parameter	TSH	M22
logKA	−8.27 ± 0.08	−8.65 ± 0.21
logKB	−7.43 ± 0.12	−7.69 ± 0.22
logτA	0.70 ± 0.03	0.59 ± 0.06

K_A, K_B and τ_A values were determined by fitting the data of Figure 6 (TSH) and Figure 7 (M22) to the operational model of allosterism. K_A is the equilibrium dissociation constant of the agonist (TSH or M22). K_B is the equilibrium dissociation constant of Org 274179-0. Log τ_A is the logarithm of the operational efficacy parameter of the agonist. For this analysis, log τ_B was fixed to −100, log α to 0, log β to −100, E_max of TSH to 100, and E_max of M22 to 120, because the E_max of M22 was 83% of that of TSH. Data are the mean ± SEM of three separate experiments.