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Schematic workflow for protein extraction protocols. Four different protocols were used for the extraction of proteins from a forest soil and a potting soil, while for the described spiking experiment procedures b) and c) were used. Abbreviations: TCA (Trichloric acid), ESI (Electron spray ionisation), MS (mass spectroscopy), RP (reversed phase), SDS (sodium dodecyl sulfate), PVPP (polyvinylpolypyrrolidone).
