Fig. 3.

Bar chart of number of proteins for the four different protocols (SDS, extraction with sodium dodecyl sulfate; WSP, extraction with SDS and phenol with pre-purification according to Wang et al. (2006); NaOH, extraction based on sodium hydroxide with further phenol purification; SP extraction with SDS and phenol) which were used for the extraction of proteins and the two different soil types (dark grey forest soil; light grey potting soil), to compare the total number of proteins obtained from the respective protocols with special emphasis on precipitation. (cutoff: 90% protein probability). Protein identifications were accepted when one peptide with 95% peptide probability was assigned to the respective protein.