FIG. 2.

Treatment of human epidermal equivalents with self-delivery CD44 siRNA (sd-siRNA) results in CD44 target gene inhibition. (A) Schematic depicting experimental timeline. Epidermal equivalents prepared from primary human keratinocytes were treated 6 times (squares) or 10 times (circles) with 2 μM CD44 sd-siRNA. One day after the final treatment, the equivalents were harvested and subjected to (B) RT-qPCR analysis to determine relative mRNA levels or (C) immunofluorescence to visualize protein expression. The mRNA levels reported are relative to GAPDH (reference gene). The standard errors represent qPCR triplicates of each sample. CD44 protein levels were determined with antibodies specific to CD44 (red) or keratin-10 (green). Scale bar: 50 μm.