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Perivascular cuffing in AAV2-hASM-treated NHP brains. (A) Hematoxylin and eosin-processed sections from vector-treated animals (high dose, 3-month survival) indicate the presence of cuffing (arrows) around blood vessels (asterisks); cuffs were composed of macrophage (CD68+) and microglial (Iba1+) infiltrates (B and C), as compared with PBS-treated control tissue (D–F). Scale bar: 200 μm. Color images available online at www.liebertpub.com/hum
