FIG. 2.

TK reduced EPC apoptosis induced by hypoxia. (A) Representative flow-cytometric analysis of apoptosis shows that TK treatment protects EPCs from hypoxia-induced apoptosis. Cotreatment with icatibant and LY294002 significantly blocked TK's effect. (B) Quantitative analysis of EPC apoptosis by flow cytometry. *p<0.05 vs. other groups. (C) Western blots for Akt and cleaved caspase-3. (D) Expression of hTK in EPCs after Ad.hTK transduction was confirmed by immunocytochemistry. Original magnification: 200×.