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. 2012 Mar 22;23(8):859–870. doi: 10.1089/hum.2011.123

FIG. 4.

FIG. 4.

TK administration increases the number of CD34+Flk-1+ progenitors in mice. (A) Representative FACS data, in which the CD34+Flk-1+ cells within the monocytic cell population of the blood were judged as EPCs. (B) Quantitative evaluation of EPCs by FACS analysis. (C) The effect of TK on CFU-EC numbers. After 7 days in culture, EPCs were double-stained with Dil-Ac-LDL and FITC-conjugated UEA lectin, and analyzed by fluorescent microscopy. (D) Quantitative evaluation of CFUs. (E) Representative FACS data, in which the CD34+Flk-1+ cells within heart tissue were judged as EPCs. (F) Relative percentage of CD34+Flk-1+ EPCs in heart tissues post MI. *p<0.05. (G) EPCs were identified in the peri-infarction zone. Immunofluorescent staining using CD34 (green) and Flk-1 (red) antibodies identified CD34+Flk-1+ cells. Nuclei were counterstained with DAPI (blue). Upper panels show representative photomicrographs of cross sections of Ad.hTK-injected heart; white arrows show positive staining. Lower panels show representative photomicrographs of cross sections of Ad.Null-injected heart. Original magnification: 800×. *p<0.05 vs. MI/Ad.Null; n=5.