TABLE 2.
Technique |
SPR |
BLI |
MS |
|||
---|---|---|---|---|---|---|
Instrument | Biacore 2000/3000 | Biacore T100 | ICx Nomadics–SensiQ Pioneer | ForteBio Octet RED 384 | CovalX MALDI HM2 | ESI-MS |
#Participants | 6 | 4 | 1 | 1 | 1 | 1 |
Sensor chip | CM5 | CM5 | COOH1 | Super streptavidin | N/A | N/A |
Immobilization | Amine (EDC/NHS) | Amine (EDC/NHS) | Amine (EDC/NHS) | Biotinylated protein “A” captured on super streptavidin chip | N/A | N/A |
BLI, Biolayer interferometry; ESI, electrospray ionization; N/A, not applicable. ICx Nomadics (Stillwater, OK, USA); ForteBio Octet RED 384 (Menlo Park, CA, USA).