Figure 2.

2D-PAGE gels showing spots of soluble proteins of transfected and cured Huh7 cells in the pI 4–7 range. Gels (a) and (b) correspond to proteins of transfected Huh7 cells, gels (c) and (d) correspond to proteins of cured Huh7 cells. Cells were incubated in the presence of an initial H. bilis inoculum of approximately 10³ cfu/mL. Protein spots in gels were visualised by silver staining. The spot intensities from triplicate gels of three independent experiments were determined using the Z3 computational software. Spots corresponding to proteins differentially expressed are indicated on the figure.