Table 1. PCR primers used for amplification of Escherichia coli genes, South Africa.
| Gene target* | Primer sequence, 5′ → 3′ | Size of amplification product, bp | Multiplex PCR in which primers are included | Reference |
|---|---|---|---|---|
| stx1 | CAGTTAATGTGGTGGCGAAGG | 348 | A | (10) |
| CACCAGACAATGTAACCGCTG | A | (10) | ||
| stx2 | ATCCTATTCCCGGGAGTTTACG | 584 | A | (10) |
| GCGTCATCGTATACACAGGAGC | A | (10) | ||
| eae | TCAATGCAGTTCCGTTATCAGTT | 482 | A | (11) |
| GTAAAGTCCGTTACCCCAACCTG | A | (11) | ||
| est | ATTTTTCTTTCTGTATTGTCTT | 190 | B | (12) |
| CACCCGGTACAAGCAGGATT | B | (12) | ||
| elt | GGCGACAGATTATACCGTGC | 440 | B | (12) |
| CGGTCTCTATATTCCCTGTT | B | (12) | ||
| ipaH | CTCGGCACGTTTTAATAGTCTGG | 933 | C | (11) |
| GTGGAGAGCTGAAGTTTCTCTGC | C | (11) | ||
| aat | CTGGCGAAAGACTGTATCAT | 630 | C | (13) |
| CAATGTATAGAAATCCGCTGTT | C | (13) | ||
| daaC | CAGGTCATCCGGTCAGTCGG | 212 | C | This study |
| CAATGCCACGTACAACCGGC | C | This study |
*stx, Shiga toxin; eae, intimin outer membrane protein; est, heat-stable enterotoxin; elt, heat-labile enterotoxin; ipaH, invasion protein; aat, transporter protein; daaC, accessory protein with a function in F1845 fimbriae production.