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(A,B) As determined by RNA in situ hybridization (ISH), RALDH2 and Pod1 are robustly expressed in the chick E7 epicardium (arrows) and EPDCs (arrowheads). RALDH2 is downregulated in the myocardial interstitium (asterisk, A). (C) Double IF using anti-Pod1 (green) and anti-RALDH2 (red) antibodies show RALDH2 and Pod1 protein co-expression (yellow) in chick E7 epicardium (arrow) and EPDCs (arrowheads, inset).
