Figure 3. RA activates Pod1 and WT1, but not NFATC1 or Tbx18, gene expression in cultured chick proepicardial (PE) cells and EPDCs.
(A,B) TF gene expression was assessed in isolated chick E7 EPDCs (A) and E4 PE cells (B) treated with MeOH+DMSO (Ctrl) as vehicle controls, RA, the RALDH inhibitor DEAB, or RA+DEAB. Fold change in TF gene expression was quantified by qPCR relative to the control set to 1.0. Statistical significance of observed differences relative to control was determined by Student’s t-test (n=4–7). *P•0.01, #P•0.05.