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High-performance liquid chromatography of cRGD coupling with the liposomes. (A) Free cRGD (500 μg/mL) eluted with a retention time of approximately 10 minutes. (B) Excess free cRGD after coupling with the liposomes gave the peak for free cRGD. (C) The liposome sample following the coupling step showed no significant peak for free cRGD at around 10 minutes.
Abbreviations: DXR, doxorubicin; cRGD, cyclo(Arg-Gly-Asp-D-Phe-Cys).
