Figure 3.

Characterization of small interfering RNA (siRNA) ternary complexes prepared in mixing order I: particle size (A) and zeta potential (B) of siRNA complexes with different amounts of hyaluronic acid (HA) (in charge); (C) gel retardation assay of siRNA/novel redox-responsive hyperbranched poly(amido amine) (named PCD)/HA (C1 and C2) and siRNA/polyethylenimine (PEI)/HA (C3 and C4) ternary complexes with different amounts of HA (from 4 to 20 in charge) in the absence of dithiothreitol (DTT) (C1 and C3) and in the presence of DTT (C2 and C4), the charge ratio of siRNA/polycation is equal to 1/20; (D) cellular uptake of fluorescein-labeled siRNA transfected by various siRNA formulations after incubation at 37°C for 4 hours in MCF-7 and MDA-MB-231 human breast cancer cells.

Note: The untreated cells were used as the control.