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. 2012 Jul 18;7:3837–3849. doi: 10.2147/IJN.S32676

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© 2012 Chen et al, publisher and licensee Dove Medical Press Ltd.

This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

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Confocal microscopic images of MDA-MB-231 human breast cancer cells after incubation for 4 hours with (A) fluorescein-labeled small interfering RNA (FAM-siRNA)/polyethylenimine (PEI) binary complex, (B) FAM-siRNA/novel redox-responsive hyperbranched poly(amido amine) (named PCD) binary complex, and FAM-siRNA/PCD/hyaluronic acid ternary complexes prepared by (C) mixing order I, (D) mixing order II, and (E) mixing order III.

Notes: The siRNA/polycation charge ratio is equal to 1/20 for binary complexes, and siRNA/polycation/hyaluronic acid charge ratio is equal to 1/20/16 for ternary complexes. Nuclei and lysosomes were stained with Hoechst 33258 (blue) and LysoTracker^® (red), respectively. The scale bars represent 10 μm.