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. 2012 Jul 18;7:3823–3835. doi: 10.2147/IJN.S29328

Figure 10.

Figure 10

Detection of apoptotic skov-3 cells by TUNEL assay. (A) Percentage of apoptotic cells summarized from data of TUNEL assay (n = 6). (B) Images show cells incubated with: (a) D-FPPP/Bcl-2; (b) D-NPPP/Bcl-2; (c) D-FPPP/SCR; (d) D-NPPP/SCR; (e) B-FPPP/Bcl-2; (f) B-NPPP/Bcl-2; (g) B-FPPP/SCR; (h) B-NPPP/ SCR; (i) normal culture medium (control group).

Notes: Incubation time: 96 hours; N/P = 30; Dose: 50 nM siRNA per well. *P < 0.05 compared with D-FPPP/Bcl-2; #P < 0.05 compared with B-FPPP/Bcl-2; &P < 0.05 compared with D-FPPP/SCR.

Abbreviations: DOX, doxorubicin; siRNA, small interference RNA; D-PPP, DOX loaded poly(ethylene glycol)–poly(ethylene imine)–poly(ɛ-caprolactone) micelle; B-PPP, blank poly(ethylene glycol)–poly(ethylene imine)–poly(ɛ-caprolactone) micelle; FPPP/Bcl-2, Bcl-2 siRNA loaded folate–poly(ethylene glycol)–poly(ethylene imine)–poly(ɛ-caprolactone) micelle; NPPP/Bcl-2, Bcl-2 siRNA loaded nontargeted poly(ethylene glycol)–poly(ethylene imine)–poly(ɛ-caprolactone) micelle; FPPP/ SCR, scrambled siRNA loaded folate–poly(ethylene glycol)–poly(ethylene imine)– poly(ɛ–caprolactone) micelle; NPPP/SCR, scrambled siRNA loaded nontargeted poly(ethylene glycol)–poly(ethylene imine)–poly(ɛ-caprolactone) micelle.