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. 2012 Jul 18;7:3813–3822. doi: 10.2147/IJN.S33101

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© 2012 Duan et al, publisher and licensee Dove Medical Press Ltd.

This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

PMC Copyright notice

Expression of luciferase gene by co-transfecting pDNA and siRNA (blue: antisense; red: nonsense; black: siRNA-free) into COS-7 cells (A) and HepG2 cells (B) using PSIA; cytotoxicity of polyplexes formed from PSIA-pDNA-antisense siRNA complex (blue bar), PSIA-pDNA-nonsense siRNA complex (red bar), and PSIA-pDNA complex (black bar) at various polymer to gene ratios (W/W) in COS- 7 cells (C) and HepG2 cells (D); the value ‘0’ on the x-axis refers to naked pDNA and siRNA solution or naked pDNA solution.

Notes: *P < 0.05; **P < 0.01.

Abbreviations: PSIA, polyspermine imidazole-4, 5-amide; RLU, relative light unit.

Expression of luciferase gene by co-transfecting pDNA and siRNA (blue: antisense; red: nonsense; black: siRNA-free) into COS-7 cells (A) and HepG2 cells (B) using PSIA; cytotoxicity of polyplexes formed from PSIA-pDNA-antisense siRNA complex (blue bar), PSIA-pDNA-nonsense siRNA complex (red bar), and PSIA-pDNA complex (black bar) at various polymer to gene ratios (W/W) in COS- 7 cells (C) and HepG2 cells (D); the value ‘0’ on the x-axis refers to naked pDNA and siRNA solution or naked pDNA solution.

Notes: *P < 0.05; **P < 0.01.

Abbreviations: PSIA, polyspermine imidazole-4, 5-amide; RLU, relative light unit.