Figure 5. Influence of HuR on DR5 toxicity. A. HuR level as measured by RT-qPCR after treatment with HuR siRNA or control siRNA. B. Levels of cell death after silencing HuR in MiaPaCa2 cells treated with DR5 agonist (0.06 μg/ml) as measured by Annexin V assay. C. Percentages of cell survival in four PDA cell lines after treatment with DR5 agonists, as assessed using Picogreen analysis. D. Percentages of cell survival after overexpression of HuR and treatment with DR5 agonists, as assessed using Picogreen analysis.