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. Author manuscript; available in PMC: 2012 Aug 8.
Published in final edited form as: Environ Microbiol. 2011 Oct 26;14(3):743–753. doi: 10.1111/j.1462-2920.2011.02625.x

Fig. 3. MS/MS confirms that cells grown in 1.75 M NaCl modify dolichol phosphate with a novel tetrasaccharide.

Fig. 3

A. Fragmentation of the [M-2H]2− ion peak at m/z 814.457 corresponding to C60 dolichol phosphate modified by a 711.2 Da tetrasaccharide comprising deoxyhexose, two hexoses and a sulfated or phosphorylated hexose.

B. Fragmentation of the [M-2H]2− ion peak at m/z 660.373 corresponding to C60 dolichol phosphate modified by the disaccharide precursor of the tetrasaccharide-modified species examined in (A).

In (A) and (B), the insets show the chemical structure of the glycan-charged C60 dolichol phosphate and the MS/MS fragmentation scheme of the [M-2H]2− ion. In addition, the chemical formula and exact mass are provided.

C. Product ion spectra showing SO3− and PO3− groups released from disaccharide-modified C60 dolichol phosphate from cells grown in 1.75 M NaCl. The inset shows the predicted isotopic distribution of SO3− (upper panel) and PO3− (lower panel) ions.