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. Author manuscript; available in PMC: 2012 Aug 8.
Published in final edited form as: Environ Microbiol. 2011 Oct 26;14(3):743–753. doi: 10.1111/j.1462-2920.2011.02625.x

Fig. 6.

Fig. 6

Linkage of ‘low-salinity’ trisaccharide and tetrasaccharide to S-layer glycoprotein Asn-498 differs as a function of medium salinity. A S-layer glycoprotein Asn-498-containing tryptic/GluC-generated fragment in cells grown in medium containing 3.4 M NaCl (left column) or 1.75 M NaCl (right column) is differentially modified by sulfated hexose-dihexose (upper pair of panels) or by the same glycan capped with a rhamnose (lower pair of panels), as detected by LC-ESI/MS. The monoisotopic [M+2H]2+ ion peaks are shown (arrows).