Figure 1. Map of opsin promoter-driven iNOS transgene used for generation transgenic mice with retinal iNOS-overexpression.

The 4.4 kb Acc65I/Xhol mouse opsin promoter fragment was isolated and cloned into the Acc65I/Sall sites of pCMVSport6-iNOS. The polyadenylation signal was provided by the existing SV40 polyadenylation sequence which was immediately downstream of the iNOS cDNA. To release the transgene from the vector for microinjection, a double restriction enzymatic digestion employing Acc65I and Pvul was performed.