Table 1. Primers used in this study.
Primers | Primer sequences (5′→3′) |
Ec glmM1 | GCCCATGGTGATGAGTAATCGTAAATATTTCG (underlined is NcoI site) |
Ec glmM2 | TTGGATCCTTAATGATGATGATGATGATGAACGGCTTTTACTGCATCGGCG (underlined is BamHI site) |
Msm glmM1 | TTCATATGGCTCGACTGTTCGGCAC (underlined is NdeI site) |
Msm glmM2 | CCGAATTCTATCCCTGCAGACTCAC (underlined is EcoRI site) |
Mtb glmM1 | GGCGCATATGGGTCGACTGTTTGGCAC (underlined is NdeI site) |
Mtb glmM2 | TAATGGATCCTCAGCGCGCGGTGCTCACCG (underlined is BamHI site) |
Msm glmM3 | GACTAGTGGTGTCCTCGAAGACGTGCCCATCGG (underlined is SpeI site) |
Msm glmM4 | TGCGGCCGCCTATCCCTGCAGACTCACGGATTC (underlined is NotI site) |
Msm glmM5 | ACTGGGCACCGCAGGTGTC |
Msm glmM6 | GTTCGGCGACTATCCCTGC |