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. 2004 Feb 2;4:1. doi: 10.1186/1471-213X-4-1

Figure 9.

Figure 9

Shh and Ptch expression patterns at different gestational stages in Dhcr7-/- and wild-type mice. Left panel shows the Shh immunostaining patterns using an antibody to Shh N-peptide at various gestational stages as indicated by the legend on the y-axis. There were no significant differences noted at any of the stages examined between wild-type (+/+) and knockout (-/-) embryos. At E10.5, Shh was detected in the floor plate (fp), notochord (nc) (A and B), mid-gut (mg) and the epithelial buds of lungs (lb) (C and D). Shh staining was restricted to epithelial cells in the distal region of the primordial tubes of lungs at E13.5 and E15.5. Note that proximal epithelial cells of primordial tubes were not immunostained for Shh (E and F, arrows). Shh was detected in the lung epithelial cells on E18.5, and mainly in the conducting airways in the neonates. Original magnifications: A~H, K and L are 10X, I and J are 20X. Right panel shows the patterns of Ptch IHC at various gestational stages. Immunostaining of Ptch from E13.5 to P 0 confirmed concordance with Shh expression and was not significantly different between wild-type and knockout embryos. Note that the proximal epithelial cells of primordial tubes were also not immunostained for Ptch at E13.5 lungs (arrows). Original magnifications: A~F, I and J are 10X. G and H are 20X.