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. Author manuscript; available in PMC: 2013 Apr 24.
Published in final edited form as: Biochemistry. 2012 Apr 10;51(16):3451–3459. doi: 10.1021/bi300070z

Figure 6. Activation of WT and variant FVIII by thrombin.

Figure 6

FXa generation assays were used to monitor rates of activation of WT and variant FVIII (100 nM) by 5 nM and 0.5 nM thrombin as described in “Experimental Procedures.” Solid lines represent WT (□) with 5 nM thrombin and WT (■) and the variants 372(P3-P3′)740/740(P3-P3′)372 (●), 740(P3-P3′)372 (○), 372(P3-P3′)740 (▲), 372(P3-P3′)740/R740Q (△), and 372(P3-P3′)1689 (♦) with 0.5 nM thrombin. All experiments were performed at least three separate times and average values are shown.