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. Author manuscript; available in PMC: 2013 Apr 24.
Published in final edited form as: Biochemistry. 2012 Apr 10;51(16):3451–3459. doi: 10.1021/bi300070z

TABLE 1.

Specific activity values and sequences of FVIII P3-P3' mutants

Factor VIII 372(P3-P3') Sequencea
(residues 370–375)		 740(P3-P3') Sequencea
(residues 738–743)		 1689(P3-P3') Sequencea
(residues 1687–1692)		 Specific Activityb
WT QIR↓SVA EPR↓SFS SPR↓SFQ %
100 ± 6
372(P3-P3')740 EPR↓SFS   98 ± 5
372(P3-P3')1689 SPR↓SFQ   14 ± 2
372(P3-P3')740/R740Q EPR↓SFS EPQ↓SFS   38 ± 4
372(P3-P3')740/740(P3-P3')372 EPR↓SFS QIR↓SVA 122 ± 18
740(P3-P3')372 QIR↓SVA   73 ± 2
a

Sequences for the P3-P3' residues flanking Arg372, Arg740, and Arg1689 are shown using single-letter amino acid code and specific mutations are indicated in bold type. Arrows indicate the position of the scissile bond.

b

Specific activity values are presented as a percentage ± standard deviation of the WT value.