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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Transplantation. 2012 Aug 15;94(3):211–217. doi: 10.1097/TP.0b013e318259d38e

Figure 2.

Figure 2

ER stress increases hepatocyte sensitivity to TNF-α induced cell death. Hepa-1 (a) or primary hepatocytes from B6 mice (b) cells were pre-incubated with vehicle control or Tg, followed by TNF-α stimulation. Cell death was measured at 24 or 48 h by LDH assay. In a separate experiment, Tg-treated hepa-1 cells were harvested after 15 min, 30 min, 1 h and 2 h of TNF-α stimulation. Cell lysates were subjected to Western blot analysis of p-NF-κB, IκB and β-actin (c). Representative results of 3 separate experiments.