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Summary of bulk pADPr scale up
| Rxn | PARP | Acceptor | Rxn Condition |
Total pADPr Yield |
|---|---|---|---|---|
| A | hPARP1 (1–1014) (2.2 mg, 19 nmol) |
hPARP1 (379–1014) (10 mg, 137 nmol) |
1h, 30°C | 14.7 mg |
| B | hTNKS1 (1093–1327) (24.9 mg, 889 nmol) |
- | 6h, RT | 119.2 mg |
| C | hTNKS1 (1093–1327) (150 mg, 5.36 µmol) |
hPARP1 (379–1014) (150 mg, 2.05 µmol) |
21h, RT | 405 mg |
| D | hTNKS1 (1093–1327) (12 mg, 429 nmol) |
Histone (90 mg, 4.29 µmol) |
1h, RT | 24.8 mg |
