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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Jul 1;19(8):788–796. doi: 10.1038/nsmb.2334

Figure 1.

Figure 1

Functionally important regions in TFIIE. Summary of TFIIE subunit domains showing intramolecular FeBABE cleavage and in vivo function. Structured domains in either subunit are labeled (WH = winged helix, ZR = zinc ribbon), as are positions that were linked to FeBABE in the course of this work. Shaded bars summarize functional assays shown in Supplementary Fig. 1a, b (black = essential for yeast growth, grey = partially redundant WH domains (lethal when both deleted), white = no phenotype upon mutation). Lines connecting Tfa1 and Tfa2 represent intramolecular FeBABE cleavages when FeBABE was conjugated to the indicated positions in Tfa1 (Supplementary Fig. 1c; Supplementary Table 1).