Typical traces showing the effect of intrathecal administration of (a) sarcosine, (b) ALX-1393, and (c) the combined application of ALX-1393 and strychnine, a glycine receptor antagonist, on continuous cystometrograms in cyclophosphamide (CYP)–treated rats. Arrows indicate the timing of drug administration. ALX-1393 showed inhibitory effects on CYP-induced bladder overactivity (b), while sarcosine has no apparent effects (a). Strychnine antagonized the inhibitory effect of ALX-1393 (c). Vehicle 1: distilled water. Vehicle 2: 100% dimethyl sulfoxide. Vehicle 3: saline).
IVP = intravesical pressure; i.t. = intrathecal.