Table 2.

Primers used for real-time reverse transcriptase–polymerase chain reaction

Name	Forward primer (5′–3′)	Reverse primer (5′–3′)	Size (bp)
GlyT1	GCTCTGGTCCTTGCTGTTTT	GTCTCTGCTTGGCTTTGTGG	595
GlyT2	GATGCTTCTCACGCTTGGAC	CAAACGCCCAGCAGACCTTC	344
GlyRα1	GCACCAAGCACTACAACAC	AGGACAGGATGACGATAAGC	123
GlyRα2	GAGACAGCAGTGGAACGATTC	TCCGCAGCAACTTGTTATCAG	167
GlyRα3	GCCTTCTGATTGTCATTCTGTC	CTCTGCGTGGTCATCGTAAG	109
GlyRβ	AAGGCACTGGTTACTACAC	CTGAGCACGGAGAAGATG	181
18S	GTAACCCGTTGAACCCCATT	CCATCCAATCGGTAGTAGCG	151

GlyT = glycine transporter; GlyR = glycine receptor.