Fig. 3.

Representative immunohistochemical images of Bcrp distribution in wild-type and Bcrp^−/− (negative control) mouse adrenal gland (a–f), pituitary gland (g–j) and epididymal fat (k–n). Because of an absence of gender difference in localization, only organs from female mice are shown. Perfusion-fixed, paraffin embedded organ-sections were incubated with a primary antibody against mouse Bcrp (BXP-9). DAB chromogene staining (brown) visualizes Bcrp localization. The sections were counterstained with Mayer’s hematoxylin. Bars 100 μm for adrenal gland (d, f) and 50 μm for pituitary gland (h, j) and epididymal fat (l, n). Details of Bcrp positivity in a zona glomerulosa, b zona fasciculata, c zona reticularis of the adrenal gland and corresponding negative controls (e) are indicated with a bar of 50 μm. g, k represent details of Bcrp positivity and corresponding negative controls (i, m) in pituitary gland and epididymal fat; bars in these insets 25 μm. Bcrp was located in the sinusoidal capillaries in adrenal gland cortex, in the capillaries of the pituitary gland and in adipocytes