Fig. 5.
a Western blot analysis of HEK293-eYFP- and -BCRP membrane vesicles. BCRP was detected using mouse-anti-BCRP antibody (BXP-21). A Michaelis–Menten plot (b) revealed net BCRP-mediated uptake of E1S. Vesicles were incubated with increasing concentrations of [3H]-E1S for 60 s at 37 °C in the presence of AMP or ATP. Net ATP-dependent uptake was calculated by subtraction of AMP values. Control (eYFP) vesicles showed maximum uptake of 6 pmol/mg min−1. Graph represents means ± SEM of triplicate measurements in a representative experiment