Fig. (3).

Visualization of catalase activity on polyacrylamide gels. The activity bands due to the three different Salmonella catalases were detected in native 9% polyacrylamide gels using the method of Woodbury et al. (1971). 50 µg of proteins from total cellular extracts were loaded in each lane: 1 and 2, ATCC 14028; 3 and 4, PF132 (katG::cat katN::kan); 5 and 6, PF139 (katE::cat katN::kan); 7 and 8, PF141 (katE::cat katG::scar); 9 and 10, DT104; 11 and 12, LT2; 13 and 14, S. Choleraesuis A50; 15 and 16, S. Enteritidis LK5. The odd lanes contain extracts from LSMMG grown bacteria. In the even lanes extracts from NG grown bacteria were loaded.