The desmoplasia consisting of fibroblasts, pancreatic stellate cells, lymphatic and vascular endothelial cells, immune cells, pathologic increased nerves, and ECM creates a complex tumor microenvironment that promotes pancreatic cancer development, invasion, metastasis and resistance to chemotherapy. Targeting the interaction between cancer and stroma in the pancreatic tumor microenvironment may contribute to the design of new diagnosis techniques and treatments for this disease.