Table 2.
Comparison of several reaction conditions for synthesis of S-protected thiomers in order to evaluate the influence of pH as well as the type and content of S-protecting ligand on the amount of conjugated aromatic ligand and remaining free thiol groups on the S-protected thiolated chitosans (means ± SD).
| Polymer | pH during reaction | S-protecting ligand | Amount of S-protecting ligand (mg) | Conjugated S-protecting ligand (μmol/g) | Free thiol groups (μmol/g) |
|---|---|---|---|---|---|
| TGA-MNA-340 | 6.0 | 6,6′-DTNA | 210 | 168 ± 86 | 155 ± 75 |
| TGA-MNA-660 | 5.9 | 6,6′-DTNA | 405 | 402 ± 149 | 245 ± 79 |
| TGA-MNA-980 | 6.1 | 6,6′-DTNA | 600 | 687 ± 101 | 310 ± 85 |
| TGA-MNA-340 | 7.5 | 6,6′-DTNA | 210 | 97 ± 49 | 255 ± 47 |
| TGA-MNA-660 | 7.6 | 6,6′-DTNA | 405 | 254 ± 88 | 345 ± 87 |
| TGA-MNA-980 | 7.7 | 6,6′-DTNA | 600 | 454 ± 90 | 521 ± 76 |
| TGA-MNA-340 | 7.6 | 6-MNA | 105 | 69 ± 29 | 278 ± 36 |
| TGA-MNA-660 | 7.5 | 6-MNA | 205 | 194 ± 78 | 450 ± 88 |
| TGA-MNA-980 | 7.6 | 6-MNA | 300 | 305 ± 50 | 630 ± 134 |